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. 2018 Jul 20;12:150. doi: 10.3389/fnbeh.2018.00150

FIGURE 3.

FIGURE 3

Recall of contextual and cued fear memory at the acute timepoint, 3 and 6 months after RUS. Stressed animals recalled contextual fear memories only at the acute timepoint as evident by increased freezing time compared to controls at the acute timepoint (A), but not at 3 months (B) or 6 months (C) after RUS. Panels (E–G) depict% freezing scores in the first minute of the cued memory test in absence and presence of a cue (tone) at the acute, 3- and 6-month timepoints, respectively. There was no significant freezing for all treatment groups when tested in a new context without cues (no tone) at all timepoints (D–F). After tone introduction, RUS mice showed a significant increase in % freezing at the acute timepoint, 3 and 6 months after RUS (D–F). Stressed mice displayed increased immobility time in minutes 4 and 6 of the FST when compared to a control group (G). Average immobility time in the last 4 min was increased in stressed mice compared to the control group (H). RUS didn’t alter open arm entries in the elevated plus maze test at 6 months after stress. Data in (A–C,H,I) were analyzed using a student t-test (n = 6–12), while data in (D–F) were analyzed using repeated measures Two-Way ANOVA followed by post hoc Sidak test (n = 10–12). Asterisks denote statistical significance as follows: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.