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. 2018 Jul 20;12:150. doi: 10.3389/fnbeh.2018.00150

FIGURE 4.

FIGURE 4

Effect of stress on HPA axis regulation at 6 months after RUS. Mice exposed to stress showed lower plasma levels of corticosterone (A) and ACTH (B), but not CRH (C) when compared to a control group at 6 months after RUS (n = 8–12). Representative western blot images for markers of glucocorticoid signaling in the hypothalamus (D). Quantification of mineralocorticoid Receptor (E), glucocorticoid receptor (F), FKBP5 (G), and CRH (H) levels in the hypothalamus (n = 5–6). Only the levels of FKBP5 (G) were significantly reduced after RUS. Plasma corticosterone levels were inversely correlated with the% freezing scores in the cued fear memory test (I) and the immobility time in the forced swim test (J) (n = 21). There was a positive correlation between% freezing scores in the cued fear memory test and the immobility time in the forced swim test (K). Each solid black triangle represents an animal in the stress group, while white circles represent control animals. Asterisks denote statistical significance as follows: ∗p < 0.05; ∗∗p < 0.01.