Figure 2.

RR ameliorated active EAE via inhibiting inflammation and demyelination. Mice were administrated via p.o. with vehicle (n = 8) or RR (n = 8–10) daily starting from day of EAE induction (A) or disease onset (C). Accumulative clinical scores of prevention protocol (B) or treatment protocol (D) for up till 30 dpi were calculated. Spinal cords from normal mice or EAE mice treated with vehicle or RR were obtained at 30 dpi (treatment protocol) and stained by H&E (E) and Luxol Fast Blue (LFB) (F) (scale bar, 100 μm). (G) Pathology scores of inflammation and demyelination are expressed as mean ± SEM (n = 4), *p < 0.05, **p < 0.01.