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. 2018 Jul 20;9:864. doi: 10.3389/fphys.2018.00864

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Copyright © 2018 Li, Wu, Gao, Yang, Yang and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RR reduced ONOO⁻ production in active EAE spinal cord. Spinal cords in normal mice and RR or vehicle-treated EAE mice (n = 3) at 18 dpi (treatment protocol) were obtained. ONOO⁻ levels were detected using HKYellow-AM probe. (A) The immunofluorescent images of HKYellow-AM (red) and nucleus (blue) in spinal cord at 18 dpi (scale bar, 10 μm). Western blotting was conducted to analyze the expression of 3-NT (B) and iNOS, p67^phox, p47^phox (C).