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. 2018 Jul 20;9:864. doi: 10.3389/fphys.2018.00864

Figure 5.

Figure 5

RR suppressed ONOO− generation by inhibiting NF-κB signaling both in vivo and in vitro. (A) Splenocytes from normal, RR-, or vehicle-treated EAE mice (n = 3) at 18 dpi (treatment protocol) were analyzed for the expression of p65 and phosphorylated IKKα/β, IκBα and p65 by western blot assay. Macrophage cell line, RAW264.7 was pre-incubated with RR (50 ug/mL) or sham for 1 h prior to LPS (1 ug/mL) challenge for 30 min (B–D). Western blotting was conducted to analyze the expressions of iNOS, p67phox, p47phox (B) and NF-κB signaling-associated proteins (C). The nuclear translocation of p65 was examined by immunofluorescent staining (D). Scale bar represented 5 μm.