TABLE 4.
Performance of the prototype anti-Babesia assay for detection of antibodies in the macaque model of TTB
| Phase and animal | Length of collection period (days) | Day of 1st PCR-positive resulta | Day of 1st detection |
Total no. of bleeds detected |
No. of IFA-positive bleeds detected by the anti-Babesia assay (%) | ||
|---|---|---|---|---|---|---|---|
| Anti-Babesia assay | IFA test | Anti-Babesia assay | IFA test | ||||
| Phase 1 | |||||||
| RFi9 | 207 | 35 | 42 | 49 | 19 | 18 | 18 (100) |
| RGc8 | 207 | 35 | 28b | 35b | 22 | 21 | 21 (100) |
| Phase 2 | |||||||
| RZz9 | 203 | 4 | 21 | 14 | 20 | 21 | 20 (95.2) |
| RCq10 | 203 | 4 | 7 | 14 | 22 | 21 | 21 (100) |
| RLk10 | 203 | 4 | 14 | 14 | 21 | 21 | 21 (100) |
| Phase 3 | |||||||
| RVf12 | 210 | 4 | 14 | 18 | 24 | 23 | 23 (100) |
| Total | 128 | 125 | 124 (99.2) | ||||
a
As reported in Gumber et al. (25).
b
Macaque displayed transient immune response for a single bleed on day 7 (anti-Babesia assay) and on day 14 (IFA test). These results were not included in the total calculation.