Figure 1.

Protein patterning efficiency is a function of LAP concentration. Thiolated transferrin (100 μM) and LAP (0, 10, 20, 100 μM) were swelled into allyl sulfide hydrogels and irradiated through a chrome photomask with 5 mW/cm² 365 nm light for 180 s. Hydrogels were washed with PBS and immunostained for transferrin for visualization (A). Pixel intensity was averaged for the patterned region and subtracted from the average pixel intensity of the nonpatterned region to obtain normalized pixel intensity (B). Scale bars represent 50 μm.