Figure 3.

Protein release depends on LAP concentration and light intensity. Hydrogels with patterned transferrin were swelled with 50 mM mPEG_1K-SH and LAP (0.1, 1.0, 10 mM), placed over a chrome photomask with a repeating 250 μm × 250 μm square pattern, and irradiated with 365 nm light at specific I_o (0.5, 5.0, 50 mW/cm²). Immunostaining for transferrin was used to visualize patterning (A). Percent transferrin release was calculated by measuring the pixel intensity of the released region and pixel intensity of the original patterned region, normalized to the pixel intensity of unpatterned regions (B). Scale bar represents 50 μm.