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. 2018 Jul 12;4(7):909–916. doi: 10.1021/acscentsci.8b00325

Figure 5.

Figure 5

GFP-Smad3-expressing mouse embryonic fibroblasts respond to TGF-β1 patterned hydrogels. MEFs were seeded on hydrogels patterned with TGF-β1 (10 ng/mL) and cultured for 3 h (A). TGF-β1 was released in situ via a subsequent thiol–ene reaction with 50 mM PEG thiol, and cells were cultured for an additional 90 min (B). Inset of merged image shows cells within the pattern contain the Smad3 reporter in the nucleus (C, D). Mean nuclear GFP signal was measured for cells within the patterned region, outside the patterned region, and for cells after release of TGF-β1 (E) . At least 260 cells were analyzed per condition. Letters a, b, and c denote groups that are statistically distinct (p < 0.001) according to two-way ANOVA with Bonferroni testing for multiple comparisons. Data are shown as mean ± standard error (SEM). TGF-β1 (red), DAPI (blue), Smad3 (green).