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. 2018 Jul 27;20:157. doi: 10.1186/s13075-018-1652-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Western blot analysis of COL I and FN on cultured systemic sclerosis (SSc) and healthy subject (HS) fibroblasts after 24 and 48 h of CTLA4-Ig treatment. Western blotting analysis of COL I and FN in cultured SSc and HS fibroblasts. Western blotting of COL I and FN, and related densitometric analysis on cultured SSc and HS fibroblasts either untreated (CNT) or treated for 24 h (a) and 48 h (b) with various concentrations of CTLA4-Ig (line 1: CNT, calibrator; line 2: CTLA4-Ig 10 μg/ml: line 3: CTLA4-Ig 50 μg/ml; line 4: CTLA4-Ig 100 μg/ml; line 5: CTLA4-Ig 500 μg/ml; line 6: Molecular Weight)