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Physicochemical features of PAF and its protein variants.
| PAF | PAFvar | PAFopt | |
|---|---|---|---|
| Substitution | None | T8Y S10K | T8K K9T S10K E13K |
| Charge (pH 7) | +4.7 | +5.7 | +7.7 |
| GRAVY | -1.38 | -1.44 | -1.44 |
Amino acid substitutions, the predicted net charge (at pH 7, http://protcalc.sourceforge.net) and GRAVY (www.gravy-calculator.de) are indicated.
