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. 2018 Jul 27;15:114. doi: 10.1186/s12985-018-1021-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — IFA confirms seroconversion of reovirus T3D-infected mice. IFA based on reovirus T3D- (a-d) and reovirus T2J-infected (e + f) MA104-cells probed with anti-reovirus type-3 σ-1 monoclonal antibody (mAb, a; positive control), serum of an uninfected specific pathogen-free (SPF) mouse (b; negative control) and sera of reovirus T3D-infected BALB/c (c + e) and C57BL/6 (d + f) mice. Fluorescence staining relied on an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (green). Cell nuclei were stained with DAPI (blue). Scale bar: 50 μm