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. 2018 Jul 27;15:114. doi: 10.1186/s12985-018-1021-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — SDS-PAGE (a) and immunoblots (b) of recombinant Strep-rσ-1-His after purification from inclusion bodies. a Proteins were stained with colloidal Coomassie. M denotes marker proteins with the molecular masses in kDa indicated left. b Electroblot probed with a commercial anti-reovirus type-3 serum (anti-Reo-3), serum obtained from a mouse experimentally infected with reovirus T3D (Reo-T3D [+]), and a serum from an uninfected mouse (Reo-T3D [-])