Table 1.

Diagnostic parameter of Strep-rσ-1-His indirect ELISA

Diagnostic parameter	Value
Repeatability	PC: 5.4% CoV (n = 36) NC: 6.0% CoV (n = 36)
Intermediate Precision	PC: 11.2% CoV (n = 5) NC: 15.8% CoV (n = 5)
Long-term Stability	> 70 d (4 °C); < 10 d (37 °C)
LODref (D-SN; D-SP)	0.31 (100%, n = 30; 98.2%, n = 110)
ROCref (D-SN; D-SP)	0.36 (100%, n = 30; 99.1%, n = 110)
Selectivity based on ROCref	98.8% (n = 80)

Raw data of repeatability, intermediate precision, and long-term stability are listed in the supplement (Additional file 5). Ref Calculation based on sera of experimentally infected and control SPF mice, PC positive control, NC negative control, CoV coefficient of variation