Fig. 4.

The effect of HZ08 on mitochondrial respiration. a and b, PC-3 cells were treated with 5 μM HZ08 or 6 Gy IR. Mitochondrial OCR in the cells was analyzed using a Seahorse XF-96 analyzer. Supplemental reagents including oligomycin, FCCP, rotenone were automatically injected into the analyzing plates to determine basal, ATP-linked and maximal OCR as well as background image, respectively. The OCR reverse capacity, an important mitochondrial respiration index, was calculated by the maximal OCR subtracted the basal OCR. c and d, PC-3 cells were pretreated with HZ08 at different doses as 2.5 μM (L), 5 μM (M) and 10 μM (H), and then followed by 6 Gy IR. Mitochondrial OCR in the treated cells was quantified accordingly. Mean ± SD was representative of three independent experiments carried out in duplication. *(P < 0.05), **(P < 0.01) show the significances between two groups as indicated