Fig. 7.

Validation of HZ08 effect on radiosensitization of PC-3 cells in vivo. a, 5 × 10⁶ PC-3 cells were subcutaneously injected into male nude mice to form tumors. The volumes of PC-3 bearing tumors were measured every other day. The mice were treated with HZ08 (4 mg/kg) and 5 × 3 Gy IR when the tumor volumes reached to 500 mm³. Tumor growth was observed daily until the volumes reached to 2000 mm³. b-d, Tumor tissues were homogenized and total proteins and nuclear proteins were extracted. The extracted total proteins were used to measure MnSOD activity (b) and quantify amounts of MnSOD and relating signal proteins at the upstream (c and d). The extracted nuclear proteins were used to quantify the level RelB in nuclei (c). *(P < 0.05), **(P < 0.01) show the significances between two groups as indicated