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. 2018 Jul 27;18:225. doi: 10.1186/s12906-018-2293-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Cell morphology changes of Trypanosoma cruzi by fluorescent and optical microscopy. a Cell morphology, mitochondrial membrane potential, nuclear and kinetoplast DNA of T. cruzi epimastigotes after treatment with essential oils, terpenes, or BNZ. b DNA fragmentation analysis by TUNEL assay on T.cruzi epimastigotes treated with terpenes. The preserved parasitic DNA was visualized with a blue HOECHST fluorescent probe (negative TUNEL) and the free DNA strands were observed in green (positive TUNEL). ^aDIC: Differential Interference Contrast Microscopy; ^bNT: No Treatment; ^cCarOx: caryophyllene oxide; ^dLimo: limonene; ^eBNZ: Benznidazole; ^fPC: Positive control: DAPI: cells stained with DAPI nuclear fluorescent stain observed in UV filter. MitoTracker: cells stained with MitoTracker Red CMXRos stain observed in an Excitation/Emission 579/599 (nm) filter. Photographs are representative of 10 observed fields