Fig. 10.

Effects of phenformin on EGFR phosphorylation. Western blot analysis was used to examine total (t) and phosphorylated (p) forms of EGFR in MB49, T24, and UMUC3 cells treated with phenformin at various concentrations s. a–c p-EGFR and t-EGFR. β-actin was included as a loading control. d–f Relative levels of phosphorylated EGFR. Results are mean ± SD. *P < 0.05, ^#P < 0.01 vs. control