Table 4.

Bisulfite pyrosequencing and real-time PCR primers

	Primers sequences	No. of CpG sites	Annealing temp
F1 R1Bio Sequencing	GGTATTGGATGTTTTTTTTTATAAGATTAT CCAATCCCCCACTCTTTC ATTATAGTTTTTAAGGAATATTGTG	3	56
F2 R2 Sequencing	GAAAGAGTGGGGGATTGGGATT AAAATACCTACCCTCTAACCTCTACT ACCTCTACTCTTCCA	4	60
F3 R3 Bio Sequencing	AGGGTAGGTATTTTATGGTAAATTTAGGT TATCTAAAAAACCCCACAACCTATCC ATGGTAAATTTAGGTAGAATTTT	5	60
F4 R4Bio Sequencing	AGATTGGGTGGTTAATTTAGAGT TCTAAAAAACCCCACAACCTATCC GGAAAAGAGAGGGAATTATAGATAA	6	58
F5 R5 Bio Sequencing	GGGGTAGATTGGGTGGTTAA TTATCTAAAAAACCCCACAACCTATC GAGAGGTTGTTGTTTAG	5	58
BRCA1	F 5′–TGTAGGCTCCTTTTGGTTATATCATTC–3′ R 5′–CATGCTGAAACT TCTCAACCAGAA–3′		59 °C
β- Actin	F 5′–TCC CTG GAG AAG AGC TAC GA–3′ R 5′–TGA AGG TAG TTT CGT GGA TGC–3′		59 °C

F forward, R reverse