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. 2018 Jul 26;6:68. doi: 10.1186/s40478-018-0573-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a-c Representative examples of immunolabeling for γH2AX of cortical neurons in a dissociated neuron preparation (a) and cryosections (b, c) counterstained with propidium iodide (PI) from irradiated rat (a, b) and mouse (c) at 15 days post-IR. Note the presence of typical PDDF associated with the nucleolus. d, e Rat cerebral cortex cryosections double immunolabeled for γH2AX and NeuN illustrate the specific localization of PDDF in NeuN-positive neurons at 15 days post-IR. Scale bars: a-c, e, 5 μm; d, 10 μm