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. 2018 Jul 26;24:18. doi: 10.1186/s40409-018-0155-x

Fig. 1.

Fig. 1

Detection of Loxosceles spider venom by sera from Group 1 and Group 2 by dot blot and ELISA. a Dot blot for detection of L. laeta venom incubated with serum pools from Group 1 and Group 2 (1:1000 dilution). b Representative dot blot of L. laeta venom incubated with individual serum from Group 1 (blue line, dots A1-B2) and Group 2 (black line, dots C2-H5); controls (red line): monoclonal antibody 7E4-D2 anti-rLlPLD1 (1:50,000 dilution) (dot A6), polyclonal mouse anti- L. laeta venom serum (1:10,000 dilution) (dot B6), pre-immune mouse serum (1:1000 dilution) (dot C6). c Indirect ELISA for the titration of Group 1 sera that recognize L. laeta venom. d Indirect ELISA for titration of sera from Group 2 that recognize L. laeta venom