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. 2018 Jul 26;24:18. doi: 10.1186/s40409-018-0155-x

Fig. 4.

Fig. 4

Heterophilic antibodies can detect PLD from other Loxosceles species and the venom from Sicarius spiders. A quantity of 5 μg of rLlPLD1, rLlPLD2, L. intermedia PLD (LiPLD), L. reclusa PLD (LrPLD), and L. gaucho PLD (LgPLD), together with 5 μg PLA2 of Apis mellifera venom (AmPLA2), and PLC of Bacillus cereus (BcPLC) were separated by SDS-PAGE in 12% gel and transferred to a nitrocellulose membrane. Then, each protein was detected by incubation with immunoselected IgGs from both groups at a concentration of 1 μg/mL, followed by incubated with goat anti-human HRP-IgG antibody (1:50,000 dilution), and developed with ECL. a IgG purified from Group 1 sera. b IgG purified from Group 2 sera. c Immunoblot from the venom of Sicarius, with serum pools and purified IgG antibodies of groups 1 and 2. (Left) SDS-PAGE in 12% gel of L. laeta venom and Sicarius venom stained with Coomassie blue. (Right) Immunoblot of Sicarius venom detected using serum pools from Group 1 and Group 2, and IgG antibodies purified from Group 1 and Group 2 sera