Characteristics of DCs induced from PBMCs of cancer patients. DCs were induced from the PBMCs of lung cancer patients in the presence of granulocyte macrophage colony stimulating factor and interleukin-4 following in vitro culture for 7 days. (A) Representative flow cytometry plots demonstrating the surface molecular expression on the DCs. (B) The percentage of surface molecular expression on DCs induced from 12 patients with lung cancer. (C) Morphology of the induced DCs stained with Giemsa (magnification, ×1,000). CD14, cluster of differentiation 14; DCs, dendritic cells; PBMCs, peripheral blood monocytes; HLA-DR, human leukocyte antigen-D related.