Figure 2.

(A) ΔCq of six different target miRNAs measured using miRNA extracted from the cell cytoplasm, exosomes and exosome-free supernatant obtained from HK-2 and 786-O cells and normalized to Cq for miR-16 in HK-2 whole cell miRNA. Individual data points representing extractions from different cell cultures and conditioned media are represented by individual filled circles, with the mean value shown as a light gray bar with standard deviation brackets. ΔCq between selected groups were compared to indicate any significant differences. *P<0.05 vs. HK-2 cells. (B) Exosome content of miR-15a-5p, miR-34a-5p, miR-210 and miR-155 is significantly lower than in whole cells. Data from HK-2 cells and 786-O cells pooled from values shown in Fig. 2A are shown as filled circles with the means ± SD of those values shown as unfilled bars. miR, microRNA.