Figure 4.

The effect of irradiation on DNA damage repair-related proteins in H520 cells. (A) H520 cells were irradiated at 6 Gy for 0, 0.5, 1, 2, 4 and 6 h, and their protein content was harvested. Western blot analysis of the expression of 53BP1, p-ATM and γ-H2AX was conducted. (B) H520 cells were irradiated at 6 Gy for 0, 6, 12, 24, 48, 72 and 96 h, and their protein content was harvested. Western blot analysis of the expression of 53BP1 and γ-H2AX was conducted. p-ATM, phosphorylated ataxia-telangiectasia mutated.