Figure 5.

The effect of IGF-1R knockdown on γ-H2AX levels in irradiated H520 cells. (A) IGF-1R shRNA and NC cells were irradiated at 6 Gy for 48 and 72 h, and their protein content was harvested. Western blot analysis of the expression of p-IGF1R, IGF1R and p-ATM was conducted. (B) IGF-1R shRNA and NC cells were irradiated at 6 Gy for 48 and 72 h, and their protein content was harvested. Western blot analysis of the expression of 53BP1 and γ-H2AX was conducted. IGF-1R, insulin-like growth factor-1 receptor; NC, negative control.