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The effect of IGF-1R knockdown on HIF-1α, MMP-9 and VEGFA levels in H520 cells. IGF-1R shRNA and NC cells were irradiated at 6 Gy for 48 and 72 h, and their protein content was harvested. Western blot analysis of the expression of HIF-1α, MMP-9 and VEGFA was conducted. IGF-1R, insulin-like growth factor-1 receptor; HIF-1α, hypoxia inducible factor 1 α; MMP-9, matrix metallopeptidase 9; VEGFA, vascular endothelial growth factor A; NC, negative control.
