Fig. 1. (A) Structures of the substrates used in the sulfatase/glucuronidase comparative assay: 4-methylumbelliferyl sulfate (4) and N-acetyltyramine-O,β-glucuronide (5), and in the units calculation assay: phenolphthalein (6) and phenolphthalein β-d-glucuronide (7); (B) SDS-PAGE of the crude H. pomatia lysate and the purified arylsulfatase (ArS); (C) glucuronidase activity calculated for the H. pomatia crude mixture (Sigma-Aldrich) and the purified arylsulfatase fraction.