Fig. 2. Uptake and retention of the nanoformulations in MDMs. (A) and (B) For cell uptake, MDMs were treated with 100 μM ABC or prodrug nanoformulations for 1–8 h. (B) and (D) Drug retention in MDMs was determined after an 8 h drug loading followed by half media exchanges every other day for up to 30 days. Intracellular drug concentrations and CBV-TP levels were determined. Data are expressed as mean ± SD for n = 3 samples per group. (E) Transmission electron microscopy (TEM) was performed to visualize the morphologies of the formulation-loaded MDMs after 8 h incubation with nanoformulations.
