. 2018 Jun 15;209(4):1043–1054. doi: 10.1534/genetics.117.300515

Table 5. DNA strand biases of the BPSs accumulated in the MA experiments.

	LGST		LDST		LGST/LDST			LGST		LDST		LGST/LDST
Strain	A_obs	A_exp	A_obs	A_exp	A_obs	A_exp	P^a	C_obs	C_exp	C_obs	C_exp	C_obs	C_exp	P^a
MA experiments on LB
	A:T transitions							G:C transitions
mutD5	3649	3365	3108	3392	1.17	0.99	< 0.001	2440	2402	2522	2560	0.97	0.94	0.4
mutD5 mutL	7702	7046	6450	7105	1.19	0.99	< 0.001	12194	11628	11824	12390	1.03	0.94	< 0.001
mutD5 dinB	3403	3038	2698	3063	1.26	0.99	< 0.001	2498	2374	2406	2530	1.04	0.94	0.5
mutD5 dinB umuDC	3314	2967	2644	2991	1.25	0.99	< 0.001	2599	2491	2547	2655	1.02	0.94	0.03
mutD5 lexA3	5523	5001	4520	5042	1.22	0.99	< 0.001	4694	4525	4652	4821	1.01	0.94	0.01
	A:T transversions							G:C transversions
mutD5	613	608	608	613	1.01	0.99	0.8	311	332	374	353	0.83	0.94	0.3
mutD5 mutL	881	899	924	906	0.95	0.99	0.6	299	344	412	367	0.73	0.94	0.02
mutD5 dinB	447	464	485	468	0.92	0.99	0.4	226	275	342	293	0.66	0.94	0.003
mutD5 dinB umuDC	447	489	536	494	0.83	0.99	0.06	282	295	327	314	0.86	0.94	0.5
mutD5 lexA3	643	654	670	659	0.96	0.99	0.67	408	432	484	460	0.84	0.94	0.26
MA experiments on minimal medium
			A:T transitions							G:C transitions
mutD5	967	855	750	862	1.29	0.99	< 0.001	1073	976	943	1039	1.14	0.94	0.002
mutD5 mutL	1964	1722	1496	1737	1.31	0.99	< 0.001	2555	2342	2284	2496	1.12	0.94	< 0.001
	A:T transversions							G:C transversions
mutD5	278	289	302	291	0.92	0.99	0.5	133	137	149	145	0.89	0.94	0.8
mutD5 mutL	82	85	89	86	0.92	0.99	0.7	42	48	58	51	0.72	0.94	0.4

LGST, lagging strand template; LDST, leading strand template; obs, observed; exp, expected; MA, mutation accumulation experiments.

P is the probability of the χ² value calculated comparing the observed to the expected values. The expected values were calculated from the ratios of nucleotides of each type on the LGST vs. the LDST in the MG1655 genome, which are 0.99 for adenines and 0.94 for cytosines.