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. 2018 Jun 20;209(4):1183–1195. doi: 10.1534/genetics.118.301204

Figure 4.

Figure 4

The sleep-suppressing effects of FD and population density are not attributable to DAF-3. Animals were heat shocked at 37° for 11 min and examined for SIS. FD inhibits heat-induced (A) locomotor and (B) feeding quiescence in daf-3(rf) mutants. (C) Crowding enhances the sleep-suppressing effect of FD in daf-3(rf) mutants, similar to wild type (Figure 2A). The total number of animals examined is indicated at the bottom of each bar. Error bars represent SEM (25 animals per trial). Two-way ANOVA with Sidak’s (A and B) or one-way ANOVA with Tukey’s (C) multiple comparisons test. (See also Figure S5.) * P < 0.05, ** P ≤ 0.01. ns, not significant vs. control (unless otherwise indicated by connecting bars on graph).