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. 2018 Apr 16;35(8):1855–1868. doi: 10.1093/molbev/msy075

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Novel genome architecture of Δorc5 strain. (A) Scheme for outcome of recombination between sod1 and sod2 genes to split the main chromosome (+pHV4) and generate two new chromosomes (new chr 1 and new chr 2). (B) PFGE and Southern blot confirming two new chromosomes in Δorc5 strain. Intact genomic DNA of wild isolate DS2, WT H26 and Δorc5 H1689 strains was probed with sod1 and sod2 sequences. (C) Recombination of sod1 and sod2 genes in Δorc5 strain H1689 was confirmed by end-point PCR using primers to unique sequences flanking sod1 and sod. The identity of the PCR products was validated by DNA sequencing.