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. 2018 Apr 16;35(8):1855–1868. doi: 10.1093/molbev/msy075

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — New genome architectures of Δorc5 derivatives. (A) AvrII and SfaAI digests of genomic DNA from derivatives of Δorc5 strain H1689 identifying four different genome states. Strain genotypes and genome architecture state is indicated below, polymorphic and monomorphic refer to strains with H1689-type genome rearrangements. The monomorphic Δorc5 Δorc3 strain H2202 is indicated. (B) Southern blots showing that additional genome rearrangements in derivatives of Δorc5 strain H1689 did not involve recombination of the sod gene region. Genomic DNA was digested with StyI or EcoRV and probed with sequences adjacent to sod1 or sod2, respectively (for key to restriction fragments, see fig. 3A). (C) Replication profile of Δorc5 Δorc3 strain H2202 (lane 9 in panels A and B) where the genome is in a monomorphic state. Labeled as in figure 1B, the two discontinuities in the replication profile are indicated by vertical arrows. (D) Replication profile of Δorc5 Δorc3 strain H2202 remapped to sequences corresponding to new chr 1 and new chr 2.