Table 2.
H. volcanii Strains.
| Strain | Genotype | Derivation | Use |
|---|---|---|---|
| DS2 | (Mullakhanbhai and Larsen 1975) | Wild isolate | |
| H26 | ΔpyrE2 | (Allers et al. 2004) | Standard laboratory strain |
| H53 | ΔpyrE2 ΔtrpA | (Allers et al. 2004) | Laboratory strain, trpA deletion |
| Strains with large-scale genome rearrangements | |||
| H1689 | ΔpyrE2 Δorc5 | H26 pTA1375 | Deletion of orc5, large-scale genome rearrangement |
| H1822 | ΔpyrE2 Δorc5 ΔtrpA | H1689 pTA95 | trpA deletion in Δorc5 strain |
| H2149 | ΔpyrE2 Δorc5 Δorc9 | H1689 pTA1433 | orc9 deletion in Δorc5 strain |
| H2196 | ΔpyrE2 Δorc5 Δorc1 | H1689 pTA1610 | orc1 deletion in Δorc5 strain |
| H2202 | ΔpyrE2 Δorc5 Δorc3 | H1689 pTA1373 | orc3 deletion in Δorc5 strain |
| H2313 | ΔpyrE2 Δorc5 ΔtrpA Δorc2:: trpA+ | H1822 pTA1632 | orc2 deletion in Δorc5 strain |
| H2458 | ΔpyrE2 Δorc5 Δorc3 Δorc9 | H2202 pTA1433 | orc9 deletion in Δorc5 Δorc3 strain |
| H2459 | ΔpyrE2 Δorc5 Δorc1 Δorc9 | H2196 pTA1433 | orc9 deletion in Δorc5 Δorc1 strain |
| H2562 | ΔpyrE2 Δorc5 Δorc9 Δorc2 | H2149 pTA1379 | orc2 deletion in Δorc5 Δorc9 strain |
| H2733 | ΔpyrE2 Δorc5 Δorc3 ΔtrpA | H2202 pTA95 | trpA deletion in Δorc5 Δorc3 strain |
| H2738 | ΔpyrE2 Δorc5 Δorc3 Δorc9 ΔtrpA | H2458 pTA95 | trpA deletion in Δorc5 Δorc3 Δorc9 strain |
| H2786 | ΔpyrE2 Δorc5 Δorc9 ΔtrpA | H2149 pTA95 | trpA deletion in Δorc5 Δorc9 strain |
| H3195 | ΔpyrE2 Δorc5 p.tnaA-radA+ | H1689 pTA1837 | Tryptophan-inducible radA allele in Δorc5 strain |
| Strains with wild-type genome architecture | |||
| H1691 | ΔpyrE2 Δorc2 | H26 pTA1379 | Deletion of orc2 |
| H1829 | ΔpyrE2 Δorc4:: trpA+ | H53 pTA1452 | Deletion of orc4 |
| H2197 | ΔpyrE2 Δorc1 Δorc2 | H2199 pTA1610 | orc2 deletion in Δorc1 strain |
| H2199 | ΔpyrE2 Δorc1 | H26 pTA1610 | Deletion of orc1 |
| H2203 | ΔpyrE2 Δorc2 Δorc3 | H1691 pTA1373 | orc3 deletion in Δorc2 strain |
| H2304 | ΔpyrE2 Δorc3 Δori-pHV4 | H26 pTA1631 | Deletion of ori-pHV4 and orc3 |
| H2305 | ΔpyrE2 Δorc1 Δorc2 Δorc5 | H2197 pTA1375 | orc5 deletion in Δorc1 Δorc2 strain |
| H2308 | ΔpyrE2 Δorc2 Δorc3 Δorc5 | H2203 pTA1375 | orc5 deletion in Δorc2 Δorc3 strain |
| H2312 | ΔpyrE2 Δorc2 Δorc5 | H1691 pTA1375 | orc5 deletion in Δorc2 strain |
| H2413 | ΔpyrE2 Δorc1 Δorc2 Δorc5 Δorc3 | H2305 pTA1373 | orc3 deletion in Δorc1 Δorc2 Δorc5 strain |
| H2490 | ΔpyrE2 Δorc3 Δori-pHV4 Δorc2 oriC3 | H2304 pTA1692 | oriC3 and orc2 deletion in Δori-pHV4 Δorc3 strain |
| H2492 | ΔpyrE2 Δorc2 ΔoriC3 | H26 pTA1692 | Deletion of oriC3 and orc2 |
| H2494 | ΔpyrE2 Δorc1 ΔoriC1 | H26 pTA1691 | Deletion of oriC1 and orc1 |
| H2497 | ΔpyrE2 Δorc3 Δori-pHV4 Δorc1 ΔoriC1 | H2304 pTA1691 | oriC1 and orc1 deletion in Δori-pHV4 Δorc3 strain |
| H2560 | ΔpyrE2 Δorc2 ΔoriC3 Δorc1 ΔoriC1 | H2492 pTA1691 | oriC1 and orc1 deletion in ΔoriC3 Δorc2 strain |
| H2561 | ΔpyrE2 Δorc2 ΔoriC3 Δorc3 Δori-pHV4 Δorc1 ΔoriC1 | H2490 pTA1691 | oriC1 and orc1 deletion in ΔoriC3 Δorc2 Δori-pHV4 Δorc3 strain |
| H2578 | ΔpyrE2 Δorc1 ΔoriC1 Δorc5 ΔoriC2 | H2494 pTA1712 | oriC2 and orc5 deletion in ΔoriC1 Δorc1 strain |
| H2579 | ΔpyrE2 Δorc5 ΔoriC2 | H26 pTA1712 | Deletion of oriC2 and orc5 |
| H2581 | ΔpyrE2 Δorc2 ΔoriC3 Δorc3 Δori-pHV4 Δorc5 ΔoriC2 | H2490 pTA1712 | oriC2 and orc5 deletion in ΔoriC3 Δorc2 Δori-pHV4 Δorc3 strain |
| H2656 | ΔpyrE2 Δorc1 ΔoriC1 Δorc2 ΔoriC3 Δorc3 Δori-pHV4 Δorc5 ΔoriC2 | H2561 pTA1712 | oriC2 and orc5 deletion in ΔoriC1 Δorc1 ΔoriC3 Δorc2 Δori-pHV4 Δorc3 strain |
| H2658 | ΔpyrE2 Δorc1 ΔoriC1 Δorc2 ΔoriC3 Δorc5 ΔoriC2 | H2560 pTA1712 | oriC2 and orc5 deletion in ΔoriC1 Δorc1 ΔoriC3 Δorc2 strain |
| H2729 | ΔpyrE2 Δorc3 Δori-pHV4 Δorc5 ΔoriC2 | H2579 pTA1631 | ori-pHV4 and orc3 deletion in ΔoriC2 Δorc5 strain |
| H2870 | ΔpyrE2 Δorc3 | H26 pTA1373 | Deletion of orc3 |
| H3380 | ΔpyrE2 ΔtrpA Δorc5:: trpA+ | H53 pTA1633 | Deletion of orc5 |