Table 4.
Oligonucleotides.
| Primer | Sequence (5′–3′) | Relevant Properties | Use |
|---|---|---|---|
| MHorc3F1 | CGTTCAtCGATTTGACGAGGTCATCCACG | orc3 deletion, upstream | pTA1373 |
| MHorc3R1 | GTCCCGGaTCCCGATAGATCTCGGTGTCC | orc3 deletion, upstream | pTA1373 |
| MHorc3F2 | ACGACTggATCcAGCAGTAGGTAGGTCG | orc3 deletion, downstream | pTA1373 |
| MHorc3R2 | CCTCCGtCtAGAACACGACGTGCGCGACC | orc3 deletion, downstream | pTA1373 |
| MHorc2F1 | CAGCGgTAcCGACCCGTCGCAGAGGTACG | orc2 deletion, upstream | pTA1379 |
| MHorc2R1 | CGCAGGatCCGAGGCCGCCTGACCCCACG | orc2 deletion, upstream | pTA1379 |
| MHorc2F2 | GCTCGgAtCCGGCGCATTAGCGTCGGTCC | orc2 deletion, downstream | pTA1379, pTA1692 |
| MHorc2R2 | CCGAGGTctAGACATTTCGAGGGGCGG | orc2 deletion, downstream | pTA1379, pTA1692 |
| MHorc5F1 | GTGCTAGGTacCTGAACACCCATAAGTG | orc5/oriC2orc5 deletions, downstream | pTA1375, pTA1712 |
| MHorc5R1 | GCTCGAGGATCCGGACGTGGTGAGGGACG | orc5/oriC2orc5 deletions, downstream | pTA1375, pTA1712 |
| MHorc5F2 | GTGAAGAGGaTCcTCGCTGGCGTTAGGC | orc5 deletion, upstream | pTA1375 |
| MHorc5R2 | GGGGAAtcTAGAGAACCGGAAAACCCGG | orc5 deletion, upstream | pTA1375 |
| delorc9USR | TCTTCGGGaTCCTCCCTCATCGAG | orc9 deletion, upstream | pTA1433 |
| delorc9DSF | CGGTCGgAtCCGCGCCATCTCGCTCG | orc9 deletion, downstream | pTA1433 |
| pBSR3 | ACCCCAGGCTTTACACTTTATGC | orc9 deletion, downstream | pTA1433 |
| pBSF2 | TTAAGTTGGGTAACGCCAGGG | orc9 deletion, upstream, and oriC1orc1 deletion, downstream | pTA1433, pTA1691 |
| MHorc1F1 | ACGAGCgGTaCCGGACGATGCGCGCCGGC | orc1 deletion, downstream | pTA1610 |
| dorc1DF | AGAACGggaTCCCGAAGTCCGACGC | orc1/oriC1orc1 deletion, downstream | pTA1610, pTA1691 |
| MHorc1F2 | GTTCCCGGaTCCCCTCGTGCGCCGCCTCG | orc1 deletion, upstream | pTA1610 |
| MHorc1R2 | CCACAGTCTaGaCCTCGCCGCAGTAGCCG | orc1 deletion, upstream | pTA1610 |
| oriC1-BamHL | GTACTCCGGATCCATGCTCGGTATCCG | oriC1orc1 deletion, upstream | pTA1691 |
| pBSR2 | CGCGCAATTAACCCTCACTAAAG | oriC1orc1 and oriC3orc2 deletions, upstream | pTA1691, pTA1692 |
| oriC3-BamHL | GGTGTCGGAtCcCGGCTTTCGCGTTCCG | oriC3orc2 deletion, upstream | pTA1692 |
| OriC2-BamL | CCGGTCTCGGATCCAACTTAGCTCTCACTCG | oriC2orc5 deletion, upstream | pTA1712 |
| OriC2-XbaR | CGACCCTCTAGAGCGAGGCGAGGTCGCCCC | oriC2orc5 deletion, upstream | pTA1712 |
| 5′HVO_2042_XhoI_F | cccctcgagTCTTTGCAGTCTATTTCCTTC | orc4 deletion, upstream | pID19T-HVO_2042 |
| 5′HVO_2042_HindIII_R | gggaagcttACGTGTTGCAGACCTGTATAC | orc4 deletion, upstream | pID19T-HVO_2042 |
| 3′HVO_2042_BamHI_F | cccggatccCCCACAGAACAGATGAAGTG | orc4 deletion, downstream | pID19T-HVO_2042 |
| 3′HVO_2042_XbaI_R | gggtctagaCGTGCTTCCGAGTCAGAAAC | orc4 deletion, downstream | pID19T-HVO_2042 |
| radAUSNdeR | TTCTGCCATAtgCAGTCGTTCCGCCTATACCC | p.tnaA: radA+ construct, upstream | pTA1837 |
| radAextraUS | AGACCAGCTGAGTTCCGATGGGGCTGTTC | p.tnaA: radA+ construct, upstream | pTA1837 |
| sod1F | AGTACAGGCCGAACTCGACGACGCC | sod1 Southern blot probe, diagnostic PCR and sequencing of sod1 | Figure 2B, C |
| sod1R | TCTCACGGTAACCTGTGGTCGCGCG | sod1 Southern blot probe, diagnostic PCR and sequencing of sod1 | Figure 2B, C |
| sod2F | GAAATCGCCGACGCCGTCTCGACG | sod2 Southern blot probe, diagnostic PCR and sequencing of sod2 | Figure 2B, C |
| sod2R | GAGCAGTTTCGGACCTTCGTCGGCG | sod2 Southern blot probe, diagnostic PCR and sequencing of sod2 | Figure 2B, C |
| sod1 US-left | ACAGGCTCCGAACGTATCAT | sod1U Southern blot probe | Figures 3A, 5B |
| sod1 US-right | CAGTCGGTGAGTCCCTGTAA | sod1U Southern blot probe | Figures 3A, 5B |
| sod2 DS-left | GATGACCTCCGCGACCTC | sod2D Southern blot probe | Figures 3A, 5B |
| sod2 DS-right | GGGTCGCTGAACAGGTCC | sod2D Southern blot probe | Figures 3A, 5B |