Figure 1.

Single-cell isolation. Almost all scRNA-seq methods require to dissociate cells to make a single-cell suspension. To what extend this suspension represents the cellular composition and the expression patterns of the original population is a major challenge for many tissues. In addition, using frozen samples as starting material is often not possible and can be overcome by making a suspension of nuclei instead of cells (not shown). A major difference among scRNA-seq methods is whether single wells are distributed in a controlled fashion among wells, e.g. by FACS, or randomly distributed across containers e.g using microdroplets.