Figure 2.

Two common workflows of generating scRNA-seq libraries. Many methods use oligo-dT priming, template switching, pre-amplification by PCR and tagmentation to generate libraries (left). The major other amplification method amplifies cDNA linearly using in vitro transcription (right). Early barcodes and UMIs can be introduced into the primers used for reverse transcription or for second-strand synthesis, allowing to pool reactions from many cells and to identify amplified molecules, respectively.