Table 3.
Linear regression analysis of methylation of CpG sites within the IGFBP3 gene in mononuclear cells at 9 years with maternal urinary As (GW 8, ln-transformed)
| CpG site in IGFBP3 | Location in IGFBP3 | All children (n = 112) β (95% CI); p value |
Boys (n = 49) β (95% CI); p value |
Girls (n = 63) β (95% CI); p value |
|---|---|---|---|---|
| cg08541297 | Promoter | − 0.16 (− 0.58, 0.27); 0.476 | − 0.60 (− 1.18, − 0.02); 0.042 | 0.16 (− 0.49, 0.80); 0.630 |
| cg04690927 | Promoter | 0.02 (− 0.26, 0.31); 0.880 | 0.17 (− 0.28, 0.64); 0.441 | − 0.15 (− 0.55, 0.24); 0.445 |
| cg13977557 | Promoter | 0.14 (− 0.02, 0.30); 0.087 | 0.19 (− 0.04, 0.42); 0.102 | 0.09 (− 0.14, 0.33); 0.427 |
| cg16447589 | Promoter | − 0.14 (− 0.94, 0.65); 0.722 | − 1.67 (− 2.52, − 0.81); 0.000 | 1.20 (0.01, 2.38); 0.047 |
| cg06789764 | Promoter | 0.13 (− 0.26, 0.53); 0.496 | − 0.28 (− 0.87, 0.30); 0.341 | 0.45 (− 0.11, 1.02); 0.114 |
| cg26434048 | Promoter | − 0.21 (− 0.98, 0.55); 0.581 | − 0.04 (− 1.04, 0.94); 0.921 | − 0.45 (− 1.65, 0.74); 0.450 |
| cg16875425 | Intragenic | 0.45 (− 0. 37, 1.27); 0.278 | 0.95 (− 0.28, 2.18); 0.128 | 0.19 (− 0.95, 1.32); 0.740 |
| cg03776080 | Intragenic | − 0.23 (− 0.70, 0.24); 0.342 | − 0.17 (− 0.72, 0.38); 0.534 | − 0.28 (− 1.05, 0.49); 0.473 |
| cg23193639 | Intragenic | 0.20 (− 0.27, 0.68); 0.406 | − 0.31 (− 1.04, 0.41); 0.386 | 0.58 (− 0.08, 1.24); 0.084 |
| cg22083798 | Intragenic | − 0.16 (− 0.98, 0.66); 0.700 | − 0.097 (− 1.35, 1.15); 0.877 | − 0.36 (− 1.15, 0.75); 0.521 |
| cg02120774 | Intragenic | − 0.003 (− 0.33, 0.33); 0.982 | − 0.28 (− 0.76, 0.20); 0.251 | 0.14 (− 0.34, 0.62); 0.559 |
| cg11753867 | Intragenic | 0.14 (− 0.07, 0.45); 0.193 | 0.18 (− 0.12, 0.49); 0.237 | 0.12 (− 0.18, 0.43); 0.420 |
| cg20850023 | Intragenic | − 0.06 (− 0.52, − 0.39); 0.780 | − 0.34 (− 1.01, − 0.34); 0.320 | − 0.13 (− 0.49, − 0.76); 0.674 |
Methylation data were derived from the ComBat-adjusted % methylation values (excluding sites from X and Y chromosomes for analysis on all children, and including sites from X and Y chromosomes for sex-stratified analysis). The CpG sites were selected from the sites present on IGFBP3 and were associated with prenatal exposure to As. The linear models were adjusted for child sex (except when stratified for sex), estimated granulocytes and monocytes