REAGENT or RESOURCE | SOURCE | IDENTIFIER | ||
---|---|---|---|---|
Antibodies | ||||
Chicken monoclonal anti-GFP | Abcam | Cat#13970; RRID:AB_300798 | ||
Rabbit monoclonal anti-ß-catenin | CST | Cat#8480; RRID:AB_11127855 | ||
Mouse monoclonal anti-ppERK | Sigma-Aldrich | Cat#M8159; RRID:AB_477245 | ||
Rabbit polyclonal anti-Fibronectin | Sigma-Aldrich | Cat#F3648; RRID:AB_476976 | ||
Rabbit polyclonal anti-GFP | ThermoFisher | Cat#A-6455; RRID: AB_221570 | ||
Goat anti-Chicken IgY (H+L), Alexa Fluor 488 | Invitrogen | Cat#A-11039; RRID:AB_142924 | ||
Goat anti-Mouse IgG2b, Alexa Fluor 594 | Invitrogen | Cat#A-21145; RRID:AB_2535781 | ||
Goat anti-Rabbit IgG (H+L), Alexa Fluor 594 | Invitrogen | Cat#A-11012; RRID:AB_141359 | ||
Goat anti-Rabbit IgG, Alexa Fluor 647 | Invitrogen | Cat#A-21245; RRID:AB_2535813 | ||
Anti-Digoxygenin (DIG)-AP Fab fragments | Roche | Cat#1093274; RRID:AB_2314302 | ||
Chemicals, Peptides, and Recombinant Proteins | ||||
BCI | CalbioChem | Cat#317496 | ||
SU5402 | CalbioChem | Cat#572630 | ||
BMS493 | Sigma-Aldrich | Cat#B6688 | ||
Penicillin-Streptomycin | Sigma-Aldrich | Cat#P4333 | ||
Antibiotic antimycotic solution (100 ×) | Sigma-Aldrich | Cat#A5955 | ||
L-15 medium with L-Glutamine w/o Phenol Red | GIBCO | Cat#21083–027 | ||
Fetal bovine serum (FBS) | ThermoFisher | Cat# A3160601 | ||
Fast Red tablets | Roche Diagnostics | Cat# 11496549001 | ||
Experimental Models: Organisms/Strains | ||||
Zebraf ish: Tg(β-actin:NLS-tdMCP-EGFP) | Campbell et al., 2015 | ZFIN: ZDB-TGCONSTRCT-150624–4 | ||
Zebrafish: Tg(hsp70l:dnfgfr1a-EGFP) | Lee et al., 2005 | ZFIN: ZDB-TGCONSTRCT-070117–101 | ||
Zebrafish: Tg(hsp70l:tcf7l1a-GFP) | Lewis et al., 2004 | ZFIN: ZDB-TGCONSTRCT-070117–160 | ||
Zebrafish: Tg(hsp70l:dkk1b-GFP) | Stoick-Cooper et al., 2007 | ZDB-TGCONSTRCT-070403–1 | ||
Software and Algorithms | ||||
Fiji | Schindelin et al., 2012 | https://fiji.sc/; RRID: SCR_002285 | ||
MATLAB R2016a | Mathworks |
https://www.mathworks.com/products/matlab.html; RRID: SCR_001622 |
||
Imaris 8.1.2 | Bitplane | http://www.bitplane.com/imaris/imaris; RRID:SCR_007370 | ||
GraphPad Prism 7 | GraphPad | http://www.graphpad.com/; RRID:SCR_002798 | ||
Other | ||||
Nikon A1R GaAsP inverted confocal microscope 40× 1.15 NA apo λS DIC-WI objective |
Nikon | N/A | ||
Zeiss Axio-Observer-Z1 ApoTome microscope 40× 0.75 NA dry objective |
Zeiss | N/A | ||
Leica DMI 6000 inverted microscope 10× 0.25 NA dry objective |
Leica | N/A | ||
Leica M205FA dissection microscope | Leica | N/A |