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. Author manuscript; available in PMC: 2018 Jul 27.
Published in final edited form as: Cell Rep. 2018 Jul 3;24(1):66–78.e8. doi: 10.1016/j.celrep.2018.06.023
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Chicken monoclonal anti-GFP Abcam Cat#13970; RRID:AB_300798
Rabbit monoclonal anti-ß-catenin CST Cat#8480; RRID:AB_11127855
Mouse monoclonal anti-ppERK Sigma-Aldrich Cat#M8159; RRID:AB_477245
Rabbit polyclonal anti-Fibronectin Sigma-Aldrich Cat#F3648; RRID:AB_476976
Rabbit polyclonal anti-GFP ThermoFisher Cat#A-6455; RRID: AB_221570
Goat anti-Chicken IgY (H+L), Alexa Fluor 488 Invitrogen Cat#A-11039; RRID:AB_142924
Goat anti-Mouse IgG2b, Alexa Fluor 594 Invitrogen Cat#A-21145; RRID:AB_2535781
Goat anti-Rabbit IgG (H+L), Alexa Fluor 594 Invitrogen Cat#A-11012; RRID:AB_141359
Goat anti-Rabbit IgG, Alexa Fluor 647 Invitrogen Cat#A-21245; RRID:AB_2535813
Anti-Digoxygenin (DIG)-AP Fab fragments Roche Cat#1093274; RRID:AB_2314302
Chemicals, Peptides, and Recombinant Proteins
BCI CalbioChem Cat#317496
SU5402 CalbioChem Cat#572630
BMS493 Sigma-Aldrich Cat#B6688
Penicillin-Streptomycin Sigma-Aldrich Cat#P4333
Antibiotic antimycotic solution (100 ×) Sigma-Aldrich Cat#A5955
L-15 medium with L-Glutamine w/o Phenol Red GIBCO Cat#21083–027
Fetal bovine serum (FBS) ThermoFisher Cat# A3160601
Fast Red tablets Roche Diagnostics Cat# 11496549001
Experimental Models: Organisms/Strains
Zebraf ish: Tg(β-actin:NLS-tdMCP-EGFP) Campbell et al., 2015 ZFIN: ZDB-TGCONSTRCT-150624–4
Zebrafish: Tg(hsp70l:dnfgfr1a-EGFP) Lee et al., 2005 ZFIN: ZDB-TGCONSTRCT-070117–101
Zebrafish: Tg(hsp70l:tcf7l1a-GFP) Lewis et al., 2004 ZFIN: ZDB-TGCONSTRCT-070117–160
Zebrafish: Tg(hsp70l:dkk1b-GFP) Stoick-Cooper et al., 2007 ZDB-TGCONSTRCT-070403–1
Software and Algorithms
Fiji Schindelin et al., 2012 https://fiji.sc/; RRID: SCR_002285
MATLAB R2016a Mathworks https://www.mathworks.com/products/matlab.html;
RRID: SCR_001622
Imaris 8.1.2 Bitplane http://www.bitplane.com/imaris/imaris; RRID:SCR_007370
GraphPad Prism 7 GraphPad http://www.graphpad.com/; RRID:SCR_002798
Other
Nikon A1R GaAsP inverted confocal microscope
40× 1.15 NA apo λS DIC-WI objective
Nikon N/A
Zeiss Axio-Observer-Z1 ApoTome microscope
40× 0.75 NA dry objective
Zeiss N/A
Leica DMI 6000 inverted microscope
10× 0.25 NA dry objective
Leica N/A
Leica M205FA dissection microscope Leica N/A
HHS Vulnerability Disclosure