Fig 1. Characterization of a stable eys^{rmc101/rmc101} zebrafish line.

(A) Sanger sequencing identified a five base pair deletion in exon 20 in eys^{rmc101/rmc101} zebrafish. (B) Representative gel image of RT-PCR analysis using RNA from a pool of larvae (n = 15), which shows that eys transcripts are present in both wild-type and eys^{rmc101/rmc101} zebrafish (upper panel). Sanger sequencing confirmed the presence of the five base pair deletion in the eys^{rmc101/rmc101} transcript (lower panel). (C) Protein domain structures of wild-type Eys and the truncated Eys protein that is predicted in eys^{rmc101/rmc101} zebrafish.