Figure 3. Correction of myeloid cytopenia in unconditioned WHIM mice by congenic Cxcr4^+/o BM transplantation.

(A) Cxcr4^+/o BM is superior to Cxcr4^+/+ BM for establishing durable hematopoietic chimerism in unconditioned congenic Cxcr4^+/w recipients. Experiment design is shown on the left of each row of graphs. Donor-derived cell frequencies for each Cxcr4 genotype are shown for the leukocyte subsets indicated. Data are the percentage (mean ± SEM) of total cells for each subset (n = 5–8 mice per data point). Donor genotypes are abbreviated as +/o, +/+, and +/w. See Supplemental Figure 7 for representative flow cytometry plots and transplantation conditions. (B) Correction of myeloid cytopenia. Noncompetitive transplantation of 5 × 10⁷ total BM cells from Cxcr4^+/o (red) or Cxcr4^+/w (blue) donor mice to unconditioned Cxcr4^+/w recipients. Experiment design is shown on the upper left. The lower panels show donor-derived cell frequencies for the leukocyte subsets indicated, presented as the percentage (mean ± SEM) of total cells for each subset (n = 5 mice per data point). Donor genotypes are abbreviated as +/o and +/w. The upper right 3 panels show leukocyte subset counts of total cells, recipient cells, and donor-derived cells at day 120 after transplantation of unconditioned Cxcr4^+/w mice receiving Cxcr4^+/o (red) or Cxcr4^+/w (blue) BM. Dashed lines indicate average values of blood counts for each subset of naive Cxcr4^+/+ (red, n = 58) or Cxcr4^+/w (blue, n = 38) mice in our colony. Symbols +/o→+/w and +/w→+/w are the abbreviations of Cxcr4^+/w mice receiving Cxcr4^+/o BM (red) and Cxcr4^+/w mice receiving Cxcr4^+/w BM (blue), respectively. *P < 0.05; **P < 0.01; ***P < 0.005. Single comparisons, Student’s t test; multiple comparisons, 2-way ANOVA.