Figure 5. Sox9 overexpression cancels the decrease in KRAS-driven PanIN formation following Brg1 deletion in mice.

(A) The genetic strategy used to delete Brg1 and activate oncogenic Kras and Sox9 expression in adult pancreatic acinar cells in addition to the experimental design of tamoxifen administration and analysis. (B) H&E staining and immunohistochemistry for BRG1 with Alcian blue staining, SOX9, and the HA-tag in Ptf1a-Cre^ER; Kras^G12D; Sox9OE; Brg1^fl/fl mice and Ptf1a-Cre^ER; Kras^G12D; Sox9OE; Brg1^fl/fl mice. Scale bars: 50 μm. (C) Quantification of PanINs in Ptf1a-Cre^ER; Kras^G12D; Sox9OE; Brg1^fl/+ mice and Ptf1a-Cre^ER; Kras^G12D; Sox9OE; Brg1^fl/fl mice. Red bars show incidence of BRG1-negative PanINs. n = 3 per genotype. Data are shown as mean ± SEM. Student’s t test.