Figure 2. The brains of Sec24c-deficient mice contain fewer neurons than brains of control mice.

(A) Representative images of layers II–VI in control (n = 3) and Sec24c^Nes-cKO (n = 3) mice at P0, as delineated by expression of the callosal neuron marker SATB2, the layer V marker CTIP2, and the layer VI marker TBR1. Scale bar: 100 μm. (B) Thickness of designated cortical layers (mean ± SEM) and numbers of neurons within those layers (mean ± SEM) from Sec24c^Nes-cKO (n = 3) and control (n = 3) mice. (C) Representative images of immunostained brain sections showing ISLET1/2⁺ neurons in the striatum and NKX2.1⁺ neurons in the globus pallidus of control (n = 3) and Sec24c^Nes-cKO (n = 3) mice at P0. Scale bar: 500 μm. (D) Mean area (± SEM) covered by and mean number (± SEM) of ISLET1/2⁺ and NKX2.1⁺ cells in the striatum and globus pallidus of Sec24c^Nes-cKO (n = 3) and control (n = 3) mice. GP, globus pallidus. *P < 0.05 and **P < 0.01, by Student’s t test.