Figure 4. CHD8 deletion impairs oligodendrocyte differentiation in a cell-autonomous manner.

(A) MBP immunostaining of Chd8^f/f OPCs 4 days after transduction with GFP control or Cre-GFP expressing adenovirus vectors in differentiation medium. Arrows: transduced cells. Scale bar, 100 μm.

(B) Percentage of MBP⁺ oligodendrocytes among total GFP⁺ cells after 4 days of differentiation.

(C) Diagram showing tamoxifen (TAM) administration to induce Chd8 deletion.

(D, E) Immunostaining of CHD8 (D), CC1 and MBP (E) on the corpus callosum of control and Chd8 OPC-iKO mice. Scale bars, D, 50 μm; E,100 μm.

(F) Quantification of CC1⁺ oligodendrocyte cell numbers in the corpus callosum of control and Chd8 OPC-iKO mice at P14.

(G) Percentage of MBP⁺ area in the corpus callosum of control and Chd8 OPC-iKO mice at P14.

(H, I) Immunolabeling of PDGFRα on the corpus callosum of control and Chd8 OPC-iKO mice. Scale bar, 50 μm. Panel I, quantification of PDGFRα⁺ OPCs.

n = 4 animals/genotype; *** p < 0.001, two-tailed unpaired Student’s t test in B, F, G, I.

See also Figure S4 and S5.