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. Author manuscript; available in PMC: 2018 Jul 27.
Published in final edited form as: J Cell Physiol. 2018 Jan 23;233(7):5310–5321. doi: 10.1002/jcp.26335

FIGURE 1.

FIGURE 1

Dynamics of mitochondrial swelling. Isolated cardiac mitochondria (50 µg) were incubated in the incubation buffer 100 µl buffer containing 125 mM KCl, 1 mM MgCl2, 2 mM Pi, 5 mM malate, 5 mM glutamate, 1 µM EGTA, and 20 mM Tris base, pH 7.4. The swelling was imitated by addition of 300 µM CaCl2 (3 times; 100 µM each 5min). The maximum Ca2+-induced swelling was determined after addition of 1 mM CaCl2. In the end, alamethicin (1 µM) was added and total (Ca2+-dependent and Ca2+-independent) swelling was recorded