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. 2018 Jul 27;7:e35869. doi: 10.7554/eLife.35869

Figure 3. Unitary excitatory postsynaptic potentials in PV and Sst neurons in layer 2/3 of the awake mouse barrel cortex measured together with whisker position and local field potential.

(A) Example juxtacellular recording of a presynaptic Chronos-expressing neuron. From top to bottom: Extracellular signal (Juxta), whisker angle, LFP, and light stimulus (LED). Blue/grey color boxes represent Active and Quiet states. (B and C) Time-locked individual APs evoked by a 1 ms LED stimulus during Quiet and Active states. (D) Example whole-cell recording from a PV neuron together with whisker angle, LFP and LED stimulus. (E and F) Individual uEPSP responses to 1 ms optogenetic stimuli during Quiet and Active states. (G–I) Same as panels D-F, but for a Sst neuron.

Figure 3.

Figure 3—figure supplement 1. Precisely-evoked single action potentials in excitatory neurons expressing Chronos.

Figure 3—figure supplement 1.

(A) Example single excitatory neurons expressing Chronos-eGFP in PV-Cre x LSL-tdTomato mice 24 hr post electroporation. (B) Same as in panel A, but in Sst-Cre x LSL-tdTomato mice. (C) PSTH of AP peak time in Chronos-expressing excitatory neurons during Quiet and Active states. (D) Reliable evoked single APs during Quiet and Active states. (E) Comparable short latency and small jitter of light-evoked APs across Quiet and Active states. Two-tailed Wilcoxon signed-rank test assessed statistical significance, and none was found.