| Antibodies |
| Mouse monoclonal anti-UBE3A (clone E6AP-330) |
Sigma-Aldrich |
Cat#E8655 |
| Rabbit monoclonal anti-p18 |
Cell Signaling Technology |
Cat#8975 |
| Rabbit polyclonal anti-p18 |
Sigma-Aldrich |
Cat#HPA002997 |
| Rabbit monoclonal anti-p14 |
Cell Signaling Technology |
Cat#8145 |
| Rabbit monoclonal anti-MP1 |
Cell Signaling Technology |
Cat#8168 |
| Rabbit monoclonal anti-LAMTOR4 |
Cell Signaling Technology |
Cat#12284 |
| Rabbit monoclonal anti-RagA |
Cell Signaling Technology |
Cat#4357 |
| Rabbit monoclonal anti-RagB |
Cell Signaling Technology |
Cat#8150 |
| Rabbit monoclonal anti-RagC |
Cell Signaling Technology |
Cat#5466 |
| Mouse monoclonal anti-ubiquitin |
Enzo Life Sciences |
Cat#BML-PW8810 |
| Mouse monoclonal anti-Flag |
Sigma-Aldrich |
Cat#F1804 |
| Rat monoclonal anti-LAMP2 |
Abcam |
Cat#ab13524 |
| Mouse monoclonal anti-LAMP1 |
Abcam |
Cat#ab25630 |
| Rabbit polyclonal anti-p-mTOR Ser2448 |
Cell Signaling Technology |
Cat#2971 |
| Rabbit polyclonal anti-mTOR |
Cell Signaling Technology |
Cat#2972 |
| Rabbit polyclonal anti-p-S6K1 Thr389 |
Cell Signaling Technology |
Cat#9205 |
| Rabbit polyclonal anti-S6K1 |
Cell Signaling Technology |
Cat#9202 |
| Rabbit polyclonal anti-p-S6 Ser240/244 |
Cell Signaling Technology |
Cat#2215 |
| Rabbit monoclonal anti-S6 |
Cell Signaling Technology |
Cat#2217 |
| Rabbit polyclonal anti-p-PKC |
Cell Signaling Technology |
Cat#9371 |
| Rabbit polyclonal anti-PKCα |
Cell Signaling Technology |
Cat#2056 |
| Rabbit polyclonal anti-p-4EBP1 Ser65 |
Cell Signaling Technology |
Cat#9451 |
| Rabbit monoclonal anti-4EBP1 |
Cell Signaling Technology |
Cat#9644 |
| Rabbit monoclonal anti-p-AKT Ser473 |
Cell Signaling Technology |
Cat#4060 |
| Rabbit polyclonal anti-AKT |
Cell Signaling Technology |
Cat#9272 |
| Mouse monoclonal anti-Raptor (clone 1H6.2) |
EMD Millipore |
Cat#05–1470 |
| Rabbit polyclonal anti-Rictor |
Bethyl Laboratories |
Cat#A300-459A |
| Mouse monoclonal anti-NeuN (clone A60) |
EMD Millipore |
Cat#MAB377 |
| Mouse monoclonal anti-PSD95 (clone 6G6-1C9) |
Thermo Fisher Scientific |
Cat#MA1-045 |
| Mouse monoclonal anti-Cathepsin B (clone CA10) |
EMD Millipore |
Cat#IM27L |
| Rabbit monoclonal anti-COXIV |
Cell Signaling Technology |
Cat#4850 |
| Chicken polyclonal anti-GFP |
Thermo Fisher Scientific |
Cat#A10262 |
| Mouse monoclonal anti-GAPDH (clone 6C5) |
EMD Millipore |
Cat#MAB374 |
| Mouse monoclonal anti-β-actin (clone AC-15) |
Sigma-Aldrich |
Cat#A5441 |
| Goat anti-rabbit IgG IRDye 680RD |
LI-COR Biosciences |
Cat#926–68071 |
| Goat anti-mouse IgG IRDye 800CW |
LI-COR Biosciences |
Cat#926–32210 |
| Goat anti-mouse IgG AlexaFluor 488 |
Invitrogen |
Cat#A-11029 |
| Goat anti-rabbit IgG AlexaFluor 594 |
Invitrogen |
Cat#A-11037 |
| Goat anti-rat IgG AlexaFluor 488 |
Invitrogen |
Cat#A-11006 |
| Goat anti-rat IgG AlexaFluor 594 |
Invitrogen |
Cat#A-21209 |
| Goat anti-rabbit IgG AlexaFluor 488 |
Invitrogen |
Cat#A-11008 |
| Goat anti-chicken IgY AlexaFluor 488 |
Invitrogen |
Cat#A-11039 |
| Goat anti-mouse IgG AlexaFluor 633 |
Invitrogen |
Cat#A-21052 |
| Goat anti-rabbit IgG AlexaFluor 633 |
Invitrogen |
Cat#A-21070 |
| Chemicals |
| MG132 |
EMD Millipore |
Cat#474790 |
| Bafilomycin A1 |
Sigma-Aldrich |
Cat#B1793 |
| MHY1485 |
EMD Millipore |
Cat#500554 |
| Recombinant DNA |
| HA-tagged wild-type Ube3a |
(Talis et al., 1998) |
Addgene #8648 |
| HA-tagged Ube3a-C833A |
(Talis et al., 1998) |
Addgene #8649 |
| HA-p18 |
(Bar-Peled et al., 2012) |
Addgene #42338 |
| HA-p18G2A |
(Bar-Peled et al., 2012) |
Addgene #42327 |
| Flag-p18 |
(Bar-Peled et al., 2012) |
Addgene #42331 |
| Flag-p18∆K |
This paper |
N/A |
| Flag-p18K20R |
This paper |
N/A |
| Flag-p18K31R |
This paper |
N/A |
| Flag-p18K60R |
This paper |
N/A |
| Flag-p18K103/104R |
This paper |
N/A |
| Flag-p18K151R |
This paper |
N/A |
| His-ubiquitin |
(Young et al., 2011) |
Addgene #31815 |
