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. Author manuscript; available in PMC: 2018 Jul 27.
Published in final edited form as: Cell Rep. 2018 Jul 3;24(1):155–168.e5. doi: 10.1016/j.celrep.2018.06.012

Figure 6. GBPs Mediate the Release of DN to Activate Cytosolic DNA-Sensing Pathways.

Figure 6.

(A) pJB908 plasmid extracted from cytosolic fractions of macrophages challenged with L. pneumophila (pJB908) WT or ⊿sdhA strains at 6 hpi.

(B) pJB908 plasmid extracted from cytosolic fractions of macrophages challengedwith WT L. pneumophila (pJB908) at3hpiinthe presence or absence of IFNβ 20 hr pre-treatment.

(C) Ifnb transcriptional induction from WT and L. pneumophila ⊿sdhA-infected macrophages of the indicated strains measured by qRT-PCR at 4 hpi.

(D) Cell death of B6, Casp11−/− and Casp1−/−Casp11−/− macrophages when challenged with L. pneumophila ⊿sdhA.

(E) Representative images of ⊿sdhA L. pneumophila and ASC immunofluorescence by antibody staining at 7 hpi. Images were taken with 20× lens; scale bar, 10 μm.

(F) Left: quantification of ASC-positive staining gated within regions of interest (ROIs), defined as regions of positive anti-L. pneumophila signal. Right: quantification of ASC-positive cellsfrom total numberofcells enumerated via Hoechst staining. Each symbol is a technical replicate. One representative experiment out of 3 is shown.

(G)Whole-cell lysate and precipitated supernatant from L. pneumophila-infected cells were probed by western blot for a p17 cleavage product of IL-1β Representative western blot on the left; quantification from n = 3 on the right.