REAGENT or RESOURCE Antibodies	SOURCE	IDENTIFIER
Antibodies
Anti-Caspase-11 clone 17D9	Cell Signaling Technology	14340
Anti-GBP2	Proteintech	11854–1-AP
Anti-phospho-Stat1 Y701 58D6	Cell Signaling technology	9167
Anti-STAT1	Cell signaling technology	9172
Rabbit polyclonal serum against L. pneumophila	Isberg Lab	(Laguna et al., 2006; Creasey and Isberg, 2012)
Rat polyclonal serum against L. pneumophila	Isberg Lab	(Laguna et al., 2006; Creasey and Isberg, 2012)
Anti-ASC	Adipogen	AG-25B-0006
Anti-Gapdh 14C10	Cell Signaling Technology	2118
Anti-Gapdh D4C6R	Cell Signaling Technology	97166
Anti-IFNβ (ELISA capture antibody)	Santa Cruz	sc-57201
Anti-IFNβ (ELISA detection antibody)	R&D Systems	32400–1
Bacterial and Virus Strains
L. pneumophila Lp02 ΔflaA	Isberg Lab	(Creasey and Isberg, 2012; Asrat et al., 2014)
L. pneumophila Lp02 ΔflaA ΔsdhA	Isberg Lab	(Creasey and Isberg, 2012)
L. pneumophila Lp03 (dotA-) ΔflaA	Isberg Lab	(Asrat et al., 2014)
L. pneumophila Lp02 ΔflaA Ptac:: PahpC::Gfp	Isberg Lab	(Coers et al., 2007; O’Connor et al., 2012)
L. pneumophila Lp02 ΔflaA ΔsdhA Ptac:: PahpC::Gfp	Isberg Lab	(Coers et al., 2007; O’Connor et al., 2012)
L. pneumophila Lp02 ΔflaA pJB908	Isberg Lab	(Laguna et al., 2006; Ge et al., 2012)
L. pneumophila Lp02 ΔflaA ΔsdhA pJB908	Isberg Lab	(Laguna et al., 2006; Ge et al., 2012)
Biological Samples
Human Bronchoalveolar lavage cells	Tufts Medical Center, Alexander Panda, M.D.	De-identified volunteers
Human peripheral whole blood	NY Biologics	De-identified whole blood
Chemicals, Peptides, and Recombinant Proteins
Purified NA/LE Mouse anti-mouse IFNARα/β receptor MAR1–5A3	BD PharMingen	561183
Purified NA/LE Mouse IgG1 κ Isotype control	BD PharMingen	553447
Jak inhibitor Ruxolitinib	Cayman Chemical	11609
Recombinant mouse IFNβ	PBL	12400–1
TMB substrate	ThermoFisher	N301
Deposited Data
Mi-Seq GSE110678	This paper	N/A
Experimental Models: Cell Lines
Murine bone marrow derived macrophages	Mouse long bones	N/A
Experimental Models: Organisms/Strains
C57BL/6	Jackson Laboratory	000664
Ifnar−/− (B6.129S2-Ifnar1tm1Agt/Mmjax)	Jackson Laboratory	32045-JAX
Ifnb−/−	Dr. Stephanie Vogel	N/A
GBPchr3−/−	Dr. Jorn Coers; Dr. Masahiro Yamamoto	(Yamamoto et al., 2012; Pilla et al., 2014)
Casp11−/−	Dr. Vishva Dixit	(Kayagaki et al., 2011,2013)
Casp1 −/−Casp11−/−	Jackson Laboratory	(Lietal., 1995)
Asc−/−	Dr. Kate Fitzgerald	(Zheng et al., 2011)
Oligonucleotides
Forward and reverse primers for Gbps	Sigma	(Kim et al., 2011) Table S2
Forward and reverse primers for Casp11	Sigma	(Kayagaki et al., 2011)
Murine Irf7: (F) 5’-CTTCAGCACTTTCTTCCGAGA-3’; (R) 5’-TGTAGTGTGGTGACCCTTGC-3’	Sigma	(Rocha et al., 2015) Table S4
Murine Isg15: (F) 5’-GAGCTAGAGCCTGCAGCAAT-3’; (R) 5’-TTCTGGGCAATCTGCTTCTT-3’	Sigma	(Pott et al., 2011)
Murine Mx1: (F) 5’-TCTGAGGAGAGCCAGACGAT-3’; (R) 5’-ACTCTGGTCCCCAATGACAG-3’	Sigma	(Pottetal., 2011)
human IRF7 (F) 5’-CTTGGCTCCTGAGAGGGCAG-3’; (R) 5’-CGAAGTGCTTCCAGGGCA-3’	Sigma	(Dill et al., 2014) Table S5
human ISG15 (F) 5’-TCCTGCTGGTGGTGGACAA-3’; (R) 5’ -TTGTTATTCCTCACCAGGATGCT-3’	Sigma	(Dill et al., 2014) Table S5
human Mx1 (F) 5’-GTGCATTGCAGAAGGTCAGA-3’; (R) 5’-TCAGGAGCCAGCTTAGGTGT-3’	Sigma	(Dill et al., 2014) Table S5
human GAPDH (F) 5’-GCTCCTCCTGTTCGACA GTCA-3’; (R) 5’-ACCTTCCCCATGGTGTCTGA-3’	Sigma	(Dill et al., 2014) Table S5
pJB908 plasmid amplification (F) 5’-TCAGGAA GCACAAATGTCAATG-3’; (R) 5’-GGTCTA CACCACCAAAATCACG-3’	Sigma	(Ge et al., 2012)
Primers for pUC18 flanks the M13 cloning site of the vector. (F) 5’-CAGGAAACAGCTATGAC −3’; (R) 5’-GTAAAACGACGGCCAG-3’	Sigma	Isberg Lab
Recombinant DNA
pJB908 (pMMB66EH oriRSF1010 ΔoriT tdDi bla+)	Isberg Lab	(Laguna et al., 2006)
Ptac:: PahpC::Gfp (pMMB207Dmob267)	Isberg Lab	(O’Connor et al., 2012)
Software and Algorithms
GraphPad Prism7	GraphPad	N/A
Adobe Photoshop	Adobe	N/A
Affinity Designer	Affinity	N/A