REAGENT or RESOURCE Antibodies | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-Caspase-11 clone 17D9 | Cell Signaling Technology | 14340 |
Anti-GBP2 | Proteintech | 11854–1-AP |
Anti-phospho-Stat1 Y701 58D6 | Cell Signaling technology | 9167 |
Anti-STAT1 | Cell signaling technology | 9172 |
Rabbit polyclonal serum against L. pneumophila | Isberg Lab | (Laguna et al., 2006; Creasey and Isberg, 2012) |
Rat polyclonal serum against L. pneumophila | Isberg Lab | (Laguna et al., 2006; Creasey and Isberg, 2012) |
Anti-ASC | Adipogen | AG-25B-0006 |
Anti-Gapdh 14C10 | Cell Signaling Technology | 2118 |
Anti-Gapdh D4C6R | Cell Signaling Technology | 97166 |
Anti-IFNβ (ELISA capture antibody) | Santa Cruz | sc-57201 |
Anti-IFNβ (ELISA detection antibody) | R&D Systems | 32400–1 |
Bacterial and Virus Strains | ||
L. pneumophila Lp02 ΔflaA | Isberg Lab | (Creasey and Isberg, 2012; Asrat et al., 2014) |
L. pneumophila Lp02 ΔflaA ΔsdhA | Isberg Lab | (Creasey and Isberg, 2012) |
L. pneumophila Lp03 (dotA-) ΔflaA | Isberg Lab | (Asrat et al., 2014) |
L. pneumophila Lp02 ΔflaA Ptac:: PahpC::Gfp | Isberg Lab | (Coers et al., 2007; O’Connor et al., 2012) |
L. pneumophila Lp02 ΔflaA ΔsdhA Ptac:: PahpC::Gfp | Isberg Lab | (Coers et al., 2007; O’Connor et al., 2012) |
L. pneumophila Lp02 ΔflaA pJB908 | Isberg Lab | (Laguna et al., 2006; Ge et al., 2012) |
L. pneumophila Lp02 ΔflaA ΔsdhA pJB908 | Isberg Lab | (Laguna et al., 2006; Ge et al., 2012) |
Biological Samples | ||
Human Bronchoalveolar lavage cells | Tufts Medical Center, Alexander Panda, M.D. | De-identified volunteers |
Human peripheral whole blood | NY Biologics | De-identified whole blood |
Chemicals, Peptides, and Recombinant Proteins | ||
Purified NA/LE Mouse anti-mouse IFNARα/β receptor MAR1–5A3 | BD PharMingen | 561183 |
Purified NA/LE Mouse IgG1 κ Isotype control | BD PharMingen | 553447 |
Jak inhibitor Ruxolitinib | Cayman Chemical | 11609 |
Recombinant mouse IFNβ | PBL | 12400–1 |
TMB substrate | ThermoFisher | N301 |
Deposited Data | ||
Mi-Seq GSE110678 | This paper | N/A |
Experimental Models: Cell Lines | ||
Murine bone marrow derived macrophages | Mouse long bones | N/A |
Experimental Models: Organisms/Strains | ||
C57BL/6 | Jackson Laboratory | 000664 |
Ifnar−/− (B6.129S2-Ifnar1tm1Agt/Mmjax) | Jackson Laboratory | 32045-JAX |
Ifnb−/− | Dr. Stephanie Vogel | N/A |
GBPchr3−/− | Dr. Jorn Coers; Dr. Masahiro Yamamoto | (Yamamoto et al., 2012; Pilla et al., 2014) |
Casp11−/− | Dr. Vishva Dixit | (Kayagaki et al., 2011,2013) |
Casp1 −/−Casp11−/− | Jackson Laboratory | (Lietal., 1995) |
Asc−/− | Dr. Kate Fitzgerald | (Zheng et al., 2011) |
Oligonucleotides | ||
Forward and reverse primers for Gbps | Sigma | (Kim et al., 2011) Table S2 |
Forward and reverse primers for Casp11 | Sigma | (Kayagaki et al., 2011) |
Murine Irf7: (F) 5’-CTTCAGCACTTTCTTCCGAGA-3’; (R) 5’-TGTAGTGTGGTGACCCTTGC-3’ | Sigma | (Rocha et al., 2015) Table S4 |
Murine Isg15: (F) 5’-GAGCTAGAGCCTGCAGCAAT-3’; (R) 5’-TTCTGGGCAATCTGCTTCTT-3’ | Sigma | (Pott et al., 2011) |
Murine Mx1: (F) 5’-TCTGAGGAGAGCCAGACGAT-3’; (R) 5’-ACTCTGGTCCCCAATGACAG-3’ | Sigma | (Pottetal., 2011) |
human IRF7 (F) 5’-CTTGGCTCCTGAGAGGGCAG-3’; (R) 5’-CGAAGTGCTTCCAGGGCA-3’ | Sigma | (Dill et al., 2014) Table S5 |
human ISG15 (F) 5’-TCCTGCTGGTGGTGGACAA-3’; (R) 5’ -TTGTTATTCCTCACCAGGATGCT-3’ | Sigma | (Dill et al., 2014) Table S5 |
human Mx1 (F) 5’-GTGCATTGCAGAAGGTCAGA-3’; (R) 5’-TCAGGAGCCAGCTTAGGTGT-3’ | Sigma | (Dill et al., 2014) Table S5 |
human GAPDH (F) 5’-GCTCCTCCTGTTCGACA GTCA-3’; (R) 5’-ACCTTCCCCATGGTGTCTGA-3’ | Sigma | (Dill et al., 2014) Table S5 |
pJB908 plasmid amplification (F) 5’-TCAGGAA GCACAAATGTCAATG-3’; (R) 5’-GGTCTA CACCACCAAAATCACG-3’ | Sigma | (Ge et al., 2012) |
Primers for pUC18 flanks the M13 cloning site of the vector. (F) 5’-CAGGAAACAGCTATGAC −3’; (R) 5’-GTAAAACGACGGCCAG-3’ |
Sigma | Isberg Lab |
Recombinant DNA | ||
pJB908 (pMMB66EH oriRSF1010 ΔoriT tdDi bla+) | Isberg Lab | (Laguna et al., 2006) |
Ptac:: PahpC::Gfp (pMMB207Dmob267) | Isberg Lab | (O’Connor et al., 2012) |
Software and Algorithms | ||
GraphPad Prism7 | GraphPad | N/A |
Adobe Photoshop | Adobe | N/A |
Affinity Designer | Affinity | N/A |