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. Author manuscript; available in PMC: 2019 Jul 26.
Published in final edited form as: Cell. 2018 Jul 19;174(3):521–535.e13. doi: 10.1016/j.cell.2018.06.005

Figure 2. Widespread KCC2 expression mimics the effects of CLP290 to promote functional recovery.

Figure 2

(A) Experimental scheme.

(B) Representative image stacks of longitudinal (upper) and transverse (lower) spinal cord sections, taken from the mice at 8 weeks after staggered injury, stained with anti-HA (to detect the HA-KCC2 protein). Scale bar: 500 μm (upper) and 100 μm (lower).

(C) BMS performance in experimental (AAV-PHP.B-HA-KCC2) and control (AAV-PHP.B-H2B-GFP) groups. Two-way repeated-measures ANOVA followed by post hoc Bonferroni correction. *p < 0.05.

(D) Percentage of mice that reached stepping at 8 weeks after injury.

(E and F) Quantification of bodyweight support (E) and stride length (F) at 8 weeks (n = 10 per group). Student’s t-test (two-tailed, unpaired) was applied. *p < 0.05; **p < 0.01. Error bars: SEM.

(G) Color-coded stick view decomposition of mouse right hindlimb movement during dragging (AAV-PHP.B-H2B-GFP group) and stepping (AAV-PHP.B-HA-KCC2 group).

(H) Representative right hindlimb knee and ankle angle oscillation trace and simultaneous EMG recording of mice at 8 weeks after injury. Red bars, swing and stepping; black bars, stance and dragging.

See also Figure S4 and Supplementary Movie.